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Invitrogen
N-terminal Arginylation Monoclonal Antibody (4A9), PerCP
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N-terminal Arginylation Antibody (MA5-45372) in Flow
Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with N-terminal Arginylation monoclonal antibody (Product # MA5-45372) at 1:50 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42°C for 2 hours.
产品信息
MA5-45372
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4A9
抗原
Synthetic N-terminal arginylated peptide conjugated to KLH
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:1,000 dilution of MA5-45372 was sufficient for detection of N-terminal Arginylation in 0.5 µg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
靶标信息
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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