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Agrisera
NADP-ME/NADP-malic enzyme, chloroplastic (dicots/monocots) Polyclonal Antibody
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NADP-ME/NADP-malic enzyme, chloroplastic (dicots/monocots) Antibody (AS21-4580) in WB
Protein was extracted from leaves of Arabidopsis thaliana, Oryza sativa and Neurachne alopecuroidea in protein extraction buffer (50 m M HEPES, p H 7.1,1% (w/v) polyvinylpolypyrrolidone, 1 m M EDTA, 10 m M DTT, 5 m M Mg Cl 2,1 m M PMSF) and flash frozen at -80°C. Aliquots of protein extract were thawed and denatured in SDS loading buffer at 90°C for 5 minutes. 15 µg of total protein was loaded per well and ... View More
产品信息
AS21-4580
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Flaveria trinervia NADP-ME.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Flaveria bidentis, Neurachne alopecuroidea, Neurachne muelleri, Oryza sativa, Pisum sativum, Zea mays
靶标信息
NADP-ME, also called NADP dependent malic enzyme, is a plant enzyme that decarboxylates malate to produce pyruvate, carbon dioxide, and NADPH, linking organic acid metabolism to biosynthesis and redox balance. Distinct isoforms localize to cytosol, plastids, and mitochondria, supplying NADPH for fatty acid synthesis, antioxidant systems, and stress responses while providing pyruvate for respiration and anabolic pathways. In C4 and CAM photosynthesis, plastidic NADP ME functions as a key decarboxylase in certain species, releasing carbon dioxide near Rubisco to enhance carboxylation efficiency, whereas in C3 plants NADP ME supports metabolic flexibility under variable light and environmental conditions. Regulation involves gene expression, allosteric control, and posttranslational modifications, and changes in NADP ME activity influence stomatal behavior, photorespiration support, nitrogen assimilation, and resilience to drought, salinity, and oxidative stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtNADP-ME1; AtNADP-ME2; AtNADP-ME3; AtNADP-ME4; cytoNADP-ME; NADP-dependent malic enzyme; NADP-malic enzyme; NADP-ME; OsNADP-ME2
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