Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
NEDD4L Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 3
NEDD4L Antibody (PA5-79726) in WB
Western blot analysis of NEDD4L in Lane 1: human 22RV1 whole cell lysate, Lane 2: human Jurkat whole cell lysate, Lane 3: rat pancreas tissue lysate, Lane 4: mouse pancreas tissue lysate, Lane 5: mouse NIH3T3 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, ... View More
产品信息
PA5-79726
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human NEDD4-2 recombinant protein (Position: N703-D975).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 22RV1 whole cell, human Jurkat whole cell, rat pancreas tissue, mouse pancreas tissue, mouse NIH3T3 whole cell. Flow: U20S cell.
靶标信息
NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: E3 ubiquitin-protein ligase NEDD4-like; FLJ33870; HECT-type E3 ubiquitin transferase NED4L; Nedd4-2; NEDD4.2; neural precursor cell expressed, developmentally down-regulated gene 4b; ubiquitin-protein ligase Rsp5
基因别名: 1300012C07Rik; hNEDD4-2; KIAA0439; NEDD4-2; NEDD4.2; Nedd4b; NEDD4L; NEDL3; RSP5
UniProt ID: (Human) Q96PU5
Entrez Gene ID: (Human) 23327, (Rat) 291553, (Mouse) 83814
ubiquitin-protein transferase activity
protein binding
potassium channel regulator activity
ligase activity
sodium channel regulator activity
potassium channel inhibitor activity
sodium channel inhibitor activity
ion channel binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
protein polyubiquitination
regulation of membrane depolarization
protein monoubiquitination
sodium ion transport
cellular sodium ion homeostasis
excretion
response to metal ion
protein ubiquitination
viral life cycle
water homeostasis
ion transmembrane transport
regulation of ion transmembrane transport
regulation of protein catabolic process
regulation of membrane potential
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of protein catabolic process
positive regulation of endocytosis
regulation of membrane repolarization
protein K48-linked ubiquitination
ventricular cardiac muscle cell action potential
regulation of potassium ion transmembrane transporter activity
negative regulation of potassium ion transmembrane transporter activity
negative regulation of potassium ion transmembrane transport
negative regulation of sodium ion transmembrane transport
positive regulation of dendrite extension
negative regulation of protein localization to cell surface
negative regulation of sodium ion transmembrane transporter activity
positive regulation of caveolin-mediated endocytosis
negative regulation of systemic arterial blood pressure
response to salt stress
positive regulation of sodium ion transport
negative regulation of sodium ion transport
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
regulation of bicellular tight junction assembly
positive regulation of cation channel activity
