Invitrogen

NEFL Monoclonal Antibody (6H112)

Name: NEFL Monoclonal Antibody (6H112)
Brand: Invitrogen
SKU: MA5-47465

查看其他33个NF-L抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

NEFL Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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NEFL Antibody (MA5-47465) in IHC (PFA)

Immunohistochemistry analysis of NEFL in rat frontal cortex section. Samples were stained with NEFL monoclonal antibody (Product # MA5-47465) using a dilution of 1:2,000 (green). Costained with rabbit pAb to FOX3/NeuN (Product # PA5-143605) dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections. T... View More

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产品信息

MA5-47465

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:1,000

免疫细胞化学 (ICC/IF)

1:1,000

产品规格

种属反应

Bovine, Human, Mouse, Pig, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

6H112

抗原

C-terminal peptide of human NF-L protein, GEEEDTKESEEEEKKEESAGEEQVAKKKD with an N-terminal C for coupling to KLH

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS with 50% glycerol

内含物

5mM sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2942447

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; N-FL; NEF3; NEFL; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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基因别名: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110