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Invitrogen
NF-H Polyclonal Antibody
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FIGURE: 1 / 7
NF-H Antibody (PA3-16753) in ICC/IF
Immunocytochemistry analysis of NF-H in mixed rat neuron/glial cultures. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753). Neurofilament Light (NF-L) antibody [green] and Neurofilament Heavy (NF-H) antibody. Blue is a DNA stain. NF-H antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.
Product Details
PA3-16753
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Horse,
Cat,
Human,
Mouse,
Pig,
Rat
Published species
C. elegans,
Mouse
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Native NF-H (phosphorylated) purified from bovine spinal cord.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
IgY fraction
Storage buffer
tissue culture supernatant
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; heavy neurofilament protein; intermediate filament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC
Gene Aliases: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH
UniProt ID: (Human) P12036, (Mouse) P19246
Entrez Gene ID: (Dog) 442940, (Horse) 100064328, (Human) 4744, (Pig) 100156492, (Rat) 24587, (Mouse) 380684
microtubule cytoskeleton organization
ovarian follicle atresia
axonogenesis
spinal cord development
hippocampus development
cerebral cortex development
cell projection assembly
axon regeneration
neurofilament bundle assembly
cellular response to oxidative stress
response to cocaine
peripheral nervous system neuron axonogenesis
axon development
cellular response to estradiol stimulus
regulation of organelle transport along microtubule
response to sodium arsenite
response to acrylamide
brain development
embryo development
neuron projection development
intermediate filament cytoskeleton organization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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