Invitrogen

NFAT5 Polyclonal Antibody

Name: NFAT5 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-023

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32个已发表图片

38 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

NFAT5 Antibody in Immunocytochemistry (ICC/IF)

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NFAT5 Antibody (PA1-023) in ICC/IF

Immunofluorescent analysis of NFAT5 using anti-NFAT5 polyclonal antibody (Product # PA1-023) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing NFAT5 (Product # PA1-023), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goa... View More

NFAT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFAT5 Antibody in Immunoprecipitation (IP)

NFAT5 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

NFAT5 Antibody — Figure 2 NFAT does not appear to be involved in latent HIV transcription in 5A8 cells. A, immunoblotting analysis of RelA and NFAT knockdowns in 5A8 cells. Negative control siRNA (lanes 1 and 6), siRNAs against RelA (lanes 2 and 7), NFATc1, NFATc2, and NFATc3 (lanes 3 and 8), NFAT5 alone (lanes 4 and 9), and all four NFATs (lanes 5 and 10) were introduced into 5A8 cells by Amaxa nucleofection twice within 48 h. Cells were then treated with DMSO (lanes 1 to 5) or 500 nM CsA (lanes 6 to 10) for 2 h, stimulated with anti-CD3 and anti-CD28 antibodies for 24 h, and collected for immunoblotting or FACS analyses. CsA reduced the levels of NFATc1 and NFATc2, which autoamplified their expressions after TCR crosslinking. B, FACS analysis of LTR-driven GFP reporter activity ( y-axis ). Values are mean +- SEM from four experiments. Reporter activity remained partially sensitive to CsA inhibition (lanes 6 to 10) but was insensitive to NFAT knockdown (lanes 3 to 5). C, characterization of 5A8-NFAT-DsRed2 cells. Cells were pretreated with DMSO ( panels 1-3 ) or 500 nM CsA for 2 h ( panel 4 ) and incubated with 20 nM PMA ( panel 2 ), or 20 nM PMA and 2 uM ionomycin ( panels 3 and 4 ) for 24 h, followed by FACS analysis. PMA alone induced activity of the GFP reporter but not the DsRed2 reporter ( panel 2 ). Ionomycin and PMA induced both reporters ( panel 3 ). CsA fully inhibited combined PMA/ionomycin induction of DsRed2 reporter but only partially suppressed GFP reporter activity to levels {KD}

Immunogen image

Immunogen Image NFAT5 Polyclonal Antibody

产品信息

PA1-023

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

3 µg

染色质免疫沉淀实验 (ChIP)

查看2篇已发表文章 2篇已发表文章

凝胶阻滞分析 (GS)

Assay-dependent

亲和纯化 (AP)

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CUT&RUN (C&R)

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产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse, Rabbit, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2152617

产品详细信息

PA1-023 detects nuclear factor of activated T-cells 5 (NFAT5) from human and mouse cells as well as recombinant human protein.

PA1-023 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, gel shift and immunocytochemistry procedures. By Western blot, this antibody detects an ~170 kDa protein representing NFAT5 in HEK293 cells transfected with the human NFAT5 gene. Immunocytochemical staining of NFAT5 in HEK293 cells transfected with the human NFAT5 gene with PA1-023 results in primarily cytoplasmic staining.

The PA1-023 immunogen is a synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. This sequence is 94% conserved in the mouse protein. PA1-023 immunizing peptide (Cat. # PEP-123) is available for use in neutralization and control experiments.

靶标信息

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: glutamine rich protein H65; NF-AT5; NFAT-like protein 1; Nuclear factor of activated T-cells 5; osmotic response element-binding protein; Rel domain-containing transcription factor NFAT5; T-cell transcription factor NFAT5; TonE-binding protein; tonicity-responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein

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基因别名: AI225870; B130038B15Rik; CAG-8; CAG80; KIAA0827; mKIAA0827; NF-AT5; NFAT5; NFATL1; NFATZ; OREBP; TONEBP

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UniProt ID: (Human) O94916, (Mouse) Q9WV30

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Entrez Gene ID: (Human) 10725, (Mouse) 54446

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding DNA binding Rel homology transcription factor

参与通路

cytokine production transcription from RNA polymerase II promoter response to osmotic stress signal transduction excretion positive regulation of transcription from RNA polymerase II promoter regulation of calcineurin-NFAT signaling cascade transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of gene expression

NFAT5 Polyclonal Antibody

NFAT5 Antibody (PA1-023) in ICC/IF

产品信息

PA1-023

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

凝胶阻滞分析 (GS)

亲和纯化 (AP)

CUT&RUN (C&R)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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NFAT5 Polyclonal Antibody

NFAT5 Antibody (PA1-023) in ICC/IF

产品信息

PA1-023

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

凝胶阻滞分析 (GS)

亲和纯化 (AP)

CUT&RUN (C&R)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)