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Invitrogen
NFU1 Polyclonal Antibody
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NFU1 Antibody (PA5-96576) in WB
Western blot analysis of NFU1 in extracts of various cell lines. Samples were incubated with NFU1 Polyclonal antibody (Product # PA5-96576) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-96576
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NFU1 (NP_0010027551)
偶联物
形式
Liquid
浓度
0.36 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MAATARRGWG AAAVAAGLRR RFCHMLKNPY TIKKQPLHQF VQRPLFPLPA AFYHPVRYMF IQTQDTPNPN SLKFIPGKPV LETRTMDFPT PAAAFRSPLA RQLFRIEGVK SVFFGPDFIT VTKENEELDW NLLKPDIYAT IMDFFASGLP LVTEETPSGE AGSEEDDEVV AMIKELLDTR IRPTVQEDGG DVIYKGFEDG IVQLKLQGSC TSCPSSIITL KNGIQNMLQF; Positive Samples: BT-474, SW480, THP-1, U-937, Mouse liver, Mouse heart, Mouse skeletal muscle, Rat liver, Rat heart; Cellular Location: Cytoplasm, Mitochondrion, cytosol
靶标信息
This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HIRA-interacting protein 5; histone cell cycle regulation defective interacting protein 5; iron-sulfur cluster scaffold protein; mHIRIP5; NFU1 iron-sulfur cluster scaffold homolog, mitochondrial; NifU-like C-terminal domain containing
基因别名: 0610006G17Rik; CGI-33; HIRIP; HIRIP5; MMDS1; Nfu; NFU1; NifU; NIFUC
UniProt ID: (Human) Q9UMS0, (Mouse) Q9QZ23
Entrez Gene ID: (Human) 27247, (Rat) 297416, (Mouse) 56748
