Invitrogen

NFYA Recombinant Rabbit Monoclonal Antibody (JE54-87)

Name: NFYA Recombinant Rabbit Monoclonal Antibody (JE54-87)
Brand: Invitrogen
SKU: MA5-36198

查看其他14个NFYA抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (13)

NFYA Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

NFYA Antibody (MA5-36198) in ICC/IF

Immunofluorescence analysis of NFYA was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NFYA Recombinant Rabbit Monoclonal Antibody (JE54-87) (Product # MA5-36198) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More

NFYA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image NFYA Recombinant Rabbit Monoclonal Antibody (JE54-87)

产品信息

MA5-36198

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE54-87

抗原

Synthetic peptide within Human NFYA aa 298-347

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2890476

靶标信息

The NFYA gene encodes for one of the subunits of the Nuclear Factor Y (NF-Y) transcription factor, which plays a critical role in the regulation of various genes, particularly those containing the CCAAT box within their promoter regions. Structurally, NF-Y consists of a heterotrimeric complex made up of NF-YA, NF-YB, and NF-YC subunits. NF-YA serves as the sequence-specific DNA-binding component, whereas the NF-YB/NF-YC heterodimer forms a Histone Fold Domain crucial for DNA binding and stabilization. Functionally, NF-YA is pivotal in cell cycle regulation and neuronal development, influencing neural progenitor maintenance during brain morphogenesis. It has been implicated in various physiological processes, including response to plant stress and cancer progression. Specifically, NFYA gene activity is prevalent in proliferating cells and is often dysregulated in cancerous tissues, which highlights its potential as a therapeutic target. NFYA's expression in plant systems, such as in the Arabidopsis species, is modulated by environmental stresses like drought, indicating its broader impact on biological stress responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; CAAT-box DNA-binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit A; CCAAT-binding transcription factor subunit B; FLJ11236; HAP2 CCAAT-binding protein; nf-y; NF-YA; Nuclear transcription factor Y subunit A; Nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit

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基因别名: AA407810; CBF-A; CBF-B; HAP2; NF-YA; NFYA; SEZ-10; SEZ10

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UniProt ID: (Human) P23511, (Mouse) P23708, (Rat) P18576

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Entrez Gene ID: (Human) 4800, (Mouse) 18044, (Rat) 29508

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