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Invitrogen
NIT1 Polyclonal Antibody
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NIT1 Antibody (PA5-113056) in ICC/IF
Immunocytochemichal analysis of NIT1 in HepG2 cells using a Polyclonal antibody (Product # PA5-113056) at a dilution of 1:400. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
产品信息
PA5-113056
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Deaminated glutathione amidase protein (1-243AA)
偶联物
形式
Liquid
浓度
3 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Deaminated glutathione amidase; dGSH amidase; MGC57670; Nitrilase homolog 1
基因别名: AI255805; ESTM30; NIT1; rCG_20134; W57327
UniProt ID: (Human) Q86X76, (Rat) Q7TQ94, (Mouse) Q8VDK1
Entrez Gene ID: (Human) 4817, (Rat) 289222, (Mouse) 27045
