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Invitrogen
NLRC4 Polyclonal Antibody
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NLRC4 Antibody (PA5-79739) in ICC/IF
Immunofluorescence analysis of NLRC4 (NLR family CARD domain-containing protein 4) was performed using log phase THP-1 and Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NLRC4 Polyclonal Antibody (Product # PA5-79739) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, A... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79739
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12 (838-874aa KILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDR).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: 22RV1 whole cell, RH35 whole cell, NIH3T3 whole cell.
靶标信息
NLRC4 encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CARD protein; CARD, LRR, and NACHT-containing protein; CARD12; CARD12; CED4/Apaf-1 family member; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CED-4-like protein Clan; ice protease-activating factor; ICE-protease activating factor; IPAF; CED4/Apaf-1 family member; NOD-like receptor C4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4; unnamed protein product
基因别名: 9530011P19Rik; AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
UniProt ID: (Human) Q9NPP4, (Rat) F1M649, (Mouse) Q3UP24
Entrez Gene ID: (Human) 58484, (Rat) 298784, (Mouse) 268973
activation of innate immune response
inflammatory response
positive regulation of protein processing
detection of bacterium
positive regulation of interleukin-1 beta production
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
protein homooligomerization
pyroptosis
pattern recognition receptor signaling pathway
icosanoid biosynthetic process
positive regulation of inflammatory response
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