Search
Invitrogen
NLRP3 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 5
NLRP3 Antibody (PA5-18118) in ICC/IF
Immunocytochemistry analysis of NLRP3 using NLRP3 Polyclonal Antibody (Product # PA5-18118) in paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-18118
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide sequence (EKPELTVVFEPSW) corresponding to the C-terminus amino acids of NLRP3 (aa 1024-1036).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is predicted to react with bovine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
This antibody is not recommended for WB.
靶标信息
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: caterpiller protein 1.1; cold auto-inflammatory syndrome 1; PYPAF1; NALP3; C1ORF7; cold autoinflammatory syndrome 1 protein; cold-induced autoinflammatory syndrome 1 protein; cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; FLJ95925; member of the NALP family of inflammatory mediators; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYD, NACHT, and LRR containing protein; PYRIN-containing APAF1-like protein 1; unnamed protein product
基因别名: AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; PYPAF1
UniProt ID: (Human) Q96P20
Entrez Gene ID: (Human) 114548
protein binding
ATP binding
ATPase activity
protein binding, bridging
signaling adaptor activity
identical protein binding
peptidoglycan binding
ADP binding
sequence-specific DNA binding
binding, bridging
phosphatidylinositol-4-phosphate binding
DNA-binding transcription factor binding
small molecule sensor activity
cysteine-type endopeptidase activator activity
molecular condensate scaffold activity
phosphatidylinositol phosphate binding
pattern recognition receptor signaling pathway
negative regulation of acute inflammatory response
positive regulation of type 2 immune response
apoptotic process
defense response
inflammatory response
signal transduction
osmosensory signaling pathway
detection of biotic stimulus
response to virus
negative regulation of interleukin-1 beta production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-4 production
NLRP3 inflammasome complex assembly
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
negative regulation of inflammatory response
positive regulation of inflammatory response
protein homooligomerization
protein maturation
pyroptosis
cellular response to lipopolysaccharide
cellular response to virus
negative regulation of NIK/NF-kappaB signaling
positive regulation of NIK/NF-kappaB signaling
positive regulation of T-helper 2 cell cytokine production
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
