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          • Primary Antibodies ›
          • NMT1 Antibodies

          Bethyl Laboratories

          NMT1 Polyclonal Antibody

          View all (7) NMT1 antibodies

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          Cite NMT1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          NMT1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          NMT1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          NMT1 Antibody (A304-254A) in WB

          Detection of human and mouse NMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody (Product # A304-254A) (lot A304-254A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          NMT1 Antibody in Western Blot (WB)
          NMT1 Antibody in Immunoprecipitation (IP)
          NMT1 Polyclonal Antibody

          Product Details

          A304-254A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-10 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 68 to 118 of human N-Myristoyltransferase 1.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          phosphate/tris citrate, pH 7-8

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Orangutan

          Target Information

          NMT1 is involved with Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1 MIM 139311).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: alternative, short form NMT-S; Glycylpeptide N-tetradecanoyltransferase 1; HsNMT1; long form, NMT-L; Myristoyl-CoA:protein N-myristoyltransferase 1; NMT 1; Peptide N-myristoyltransferase 1; Protein-lysine myristoyltransferase NMT1; type I N-myristoyltransferase; unnamed protein product

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          Gene Aliases: AW536594; HsNMT1; NMT; NMT1

          View more View less

          UniProt ID: (Human) P30419, (Mouse) O70310

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          Entrez Gene ID: (Human) 4836, (Mouse) 18107

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          Function(s)
          glycylpeptide N-tetradecanoyltransferase activity protein binding transferase activity transferase activity, transferring acyl groups peptidyl-lysine N6-myristoyltransferase activity myristoyltransferase activity transferase
          Process(es)
          in utero embryonic development N-terminal peptidyl-glycine N-myristoylation regulation of rhodopsin mediated signaling pathway cellular response to nutrient levels cellular ketone metabolic process protein localization to membrane positive regulation of protein localization to lysosome positive regulation of establishment of protein localization to mitochondrion positive regulation of TORC1 signaling N-terminal protein myristoylation
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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