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Invitrogen
NOLC1 Polyclonal Antibody
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图: 1 / 3
NOLC1 Antibody (PA5-106698) in ICC/IF
Immunocytochemistry analysis of NOLC1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NOLC1 polyclonal antibody (Product # PA5-106698) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-106698
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NOLC1(Accession Q14978), corresponding to amino acid residues D641-V691.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total NOLC1.
靶标信息
Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 140 kDa nucleolar phosphoprotein; HCV NS5A trans-regulated protein 13; HCV NS5A-transactivated protein 13; hepatitis C virus NS5A-transactivated protein 13; nucleolar 130 kDa protein; nucleolar and coiled-body phosphprotein 1; nucleolar phosphoprotein 140; nucleolar phosphoprotein p130; nucleolar protein p130; unnamed protein product
基因别名: NOPP130; NOPP140; NS5ATP13; P130; Srp40
UniProt ID: (Human) Q14978, (Mouse) E9Q5C9, (Rat) P41777
Entrez Gene ID: (Human) 9221, (Mouse) 70769, (Rat) 64896
RNA binding
protein kinase inhibitor activity
protein binding
protein binding, bridging
protein heterodimerization activity
molecular function inhibitor activity
TFIIB-class transcription factor binding
nuclear localization sequence binding
protein domain specific binding
box C/D snoRNA binding
box H/ACA snoRNA binding
box C/D methylation guide snoRNP complex binding
box H/ACA snoRNP complex binding
regulation of translation
nucleolus organization
positive regulation of cell proliferation
neural crest formation
neural crest cell development
box H/ACA snoRNA metabolic process
regulation of protein import into nucleus
positive regulation of transcription, DNA-templated
negative regulation of protein serine/threonine kinase activity
mitotic cell cycle
rRNA processing
ribosome biogenesis
axonogenesis
regulation of synaptic plasticity
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