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Invitrogen
NOVA1 Polyclonal Antibody
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NOVA1 Antibody (PA5-95571) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of NOVA1 in U2OS cell using NOVA1 Polyclonal Antibody (Product # PA5-95571). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL and mouse anti-Beta Tubulin antibody overnight at 4°C. DyLight 488 conjugated goat anti-rabbit IgG and DyLight 594 conjugated goat anti-mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°... View More
产品信息
PA5-95571
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human Nova1 recombinant protein (Position: T416-V509).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human PC-3 whole cell, human A549 whole cell, human U87 whole cell, human THP-1 whole cell, rat brain tissue, mouse brain tissue. IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue, human liver cancer tissue, mouse brain tissue, mouse brain tissue, rat brain tissue, rat brain tissue IHC-F: human placenta tissue. ICC/IF: A549 cell, U2OS cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; RNA-binding protein Nova-1; Ventral neuron-specific protein 1
基因别名: 9430099M15Rik; G630039L02; Nova-1; NOVA1
UniProt ID: (Human) P51513, (Mouse) Q9JKN6
Entrez Gene ID: (Human) 4857, (Mouse) 664883, (Rat) 298992
