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Invitrogen
NOX2 Polyclonal Antibody
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NOX2 Antibody (PA5-95490) in WB
Western blot analysis of NOX2 in, Lane 1: human U-87MG cell lysate, Lane 2: human Hela cell lysate, Lane 3: human HepG2 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with N... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95490
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U-87MG cell, human Hela cell, human HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet possess distinct expression patterns. gp91-phox expresses in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, with low expression in the uterus and prostate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 beta polypeptide; Cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; endothelial type gp91-phox; GP91 PHOX; gp91-1; gp91-phox; GP911; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; p91PHOX; Superoxide-generating NADPH oxidase heavy chain subunit; unnamed protein product
基因别名: AMCBX2; C88302; CGD; CGD91-phox; CGDX; CYBB; Cyd; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX
UniProt ID: (Mouse) Q61093
Entrez Gene ID: (Human) 1536, (Rat) 66021, (Mouse) 13058
protein binding
electron carrier activity
NAD(P)H oxidase activity
superoxide-generating NADPH oxidase activity
oxidoreductase activity
heme binding
voltage-gated proton channel activity
protein heterodimerization activity
flavin adenine dinucleotide binding
NADPH binding
FAD binding
superoxide-generating NADPH oxidase activity
voltage-gated ion channel activity
N-ethylmaleimide reductase activity
reduced coenzyme F420 dehydrogenase activity
sulfur oxygenase reductase activity
malolactic enzyme activity
NADPH:sulfur oxidoreductase activity
metal ion binding
epoxyqueuosine reductase activity
oxidase
superoxide metabolic process
defense response
inflammatory response
response to nutrient
response to xenobiotic stimulus
positive regulation of tumor necrosis factor production
superoxide anion generation
innate immune response
response to ethanol
respiratory burst
positive regulation of angiogenesis
cellular response to cadmium ion
cellular response to ethanol
cellular response to hypoxia
hypoxia-inducible factor-1alpha signaling pathway
response to aldosterone
cellular response to L-glutamine
response to angiotensin
response to drug
hydrogen peroxide biosynthetic process
oxidation-reduction process
transport
ion transport
regulation of ion transmembrane transport
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