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Invitrogen
NPAS4 Monoclonal Antibody (N408/79), FITC
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NPAS4 Antibody (MA5-45750) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with NPAS4 monoclonal antibody (Product # MA5-45750) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C... View More
产品信息
MA5-45750
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N408/79
抗原
Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
内含物
no preservative
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:100 dilution of MA5-45750 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 90kDa.approximately
This antibody was formerly sold as clone S408-79.
靶标信息
NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bHLH PAS type transcription factor NXF; bHLH-PAS type transcription factor NXF; bHLHe79; Class E basic helix-loop-helix protein 79; HLH-PAS transcription factor NXF; LE-PAS; Limbic-enhanced PAS protein; Neuronal PAS domain-containing protein 4; Neuronal PAS4; PAS domain-containing protein 10; PASD10 and LE-PAS
基因别名: BHLHE79; LE-PAS; NPAS4; NXF; PASD10
UniProt ID: (Human) Q8IUM7, (Rat) Q8CJH6, (Mouse) Q8BGD7
Entrez Gene ID: (Human) 266743, (Rat) 266734, (Mouse) 225872
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein heterodimerization activity
RNA polymerase II regulatory region DNA binding
DNA binding
signal transducer activity
RNA polymerase II transcription factor activity, sequence-specific DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
inhibitory postsynaptic potential
cellular response to corticosterone stimulus
transcription, DNA-templated
signal transduction
positive regulation of transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to stress
cellular response to acid chemical
