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Invitrogen
NPHP1 Polyclonal Antibody
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Product Details
PA5-93140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
2.11 mg/mL
Amount
211 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse lung, Rat brain; Cellular Location: Cell junction, Cell projection, Cytoplasm, adherens junction, cilium, cilium axoneme, cytoskeleton, tight junction
Target Information
Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway. Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells. Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Juvenile nephronophthisis 1 protein; nephrocystin 1; Nephrocystin-1; Nephrocystin1; nephronophthisis 1 (juvenile); nephronophthisis 1 (juvenile) homolog; NPH1; unnamed protein product
Gene Aliases: JBTS4; NPH1; NPHP1; SLSN1
UniProt ID: (Human) O15259, (Mouse) Q9QY53
Entrez Gene ID: (Human) 4867, (Rat) 296136, (Mouse) 53885
signal transduction
spermatogenesis
visual behavior
cell projection organization
actin cytoskeleton organization
cell differentiation
spermatid differentiation
retina development in camera-type eye
protein localization involved in establishment of planar polarity
cell-cell adhesion
positive regulation of bicellular tight junction assembly
biological_process
cellular protein localization
photoreceptor cell outer segment organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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