Invitrogen

NRF1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他19个NRF1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (9)
高级验证 (1)

NRF1 Antibody in Immunocytochemistry (ICC/IF)

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NRF1 Antibody (PA5-27854) in ICC/IF

Immunofluorescence analysis of NRF1 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NRF1 Rabbit Polyclonal Antibody (Product # PA5-27854) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:20... View More

NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NRF1 Antibody in Immunoprecipitation (IP)

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NRF1 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with NRF1 siRNA and reduction of signal was observed in Western Blot using NRF1 Polyclonal Antibody (Product # PA5-27854). {KD}

产品信息

PA5-27854

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 280 of Human NRF1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.32 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2545330

产品详细信息

Recommended positive controls: 293T, 293T nuclear, A431, A431 nuclear, HeLa, HeLa nuclear, HepG2, HepG2 nuclear.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (94%), Chicken (99%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。