Invitrogen

NRF1 Polyclonal Antibody

Name: NRF1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95573

查看其他22个NRF1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (21)

NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NRF1 Antibody (PA5-95573) in IHC (P)

Immunohistochemistry analysis of NRF1 in paraffin-embedded human gastric cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with NRF1 polyclonal antibody (Product # PA5-95573) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex a... View More

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产品信息

PA5-95573

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human NRF1 recombinant protein (Position: D246-Q503).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807375

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, human MDA-MB-231 whole cell. IHC: human placenta tissue, human ovary cancer tissue, mouse intestine tissue, mouse kidney tissue, mouse spleen tissue, rat heart tissue, rat kidney tissue, human appendicitis tissue, human gastric cancer tissue, human tonsil tissue, mouse cardiac muscle tissue, mouse liver tissue, rat brain tissue, rat intestine tissue, rat liver tissue, rat lung tissue, rat skeletal muscle tissue, rat spleen tissue. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。