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Agrisera
NRT1.1/Nitrate transporter 1.1 Polyclonal Antibody
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NRT1.1/Nitrate transporter 1.1 Antibody (AS12-2611) in WB
50 µg of microsomal protein from wild type Arabidopsis thaliana (Col 0) roots at various stages of development or from NRT 1.1 deletion mutant (CHL 1.5) were solubilized with LaemmLi x 2 sample buffer and were separated on 10% SDS-PAGE gel. The proteins were transferred (tank transfer, 100 V, 1 h) to a PVDF membrane (Immobilon-P, 0.45 um, Millipore). Blot was blocked for 2 h with agitation at room temperatu... View More
产品信息
AS12-2611
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NRT1.1 C-terminus.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
NRT1.1 encodes a plasma-membrane, proton-coupled nitrate transceptor of the plant NRT1/PTR (NPF) family in Arabidopsis thaliana, enriched in specific cell types and developmental zones including root tips and lateral root primordia as well as guard cells, consistent with roles in nutrient acquisition and signaling. The protein adopts the canonical major facilitator superfamily fold with 12 transmembrane helices organized into two six-helix bundles that form a central transport pathway; structural studies indicate it can form homodimers in the membrane, and a key regulatory phosphorylation at Thr101 (near the dimer interface) shifts the transporter between low- and high-affinity nitrate uptake modes by altering dimerization/structural flexibility. Functionally, NRT1.1 mediates nitrate influx across the plasma membrane over a wide external concentration range and also acts in nitrate sensing/signaling to shape transcriptional nitrate responses and root system architecture (notably lateral root proliferation in nitrate-rich patches); in leaves, its activity in guard cells contributes to nitrate accumulation and electrical changes associated with stomatal opening, influencing transpiration and drought responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARABIDOPSIS THALIANA NITRATE TRANSPORTER 1; AtNPF6.3; ATNRT1; ATNRT1.1; B-1; CHL1; CHL1-1; CHLORATE/NITRATE TRANSPORTER; CHLORINA 1; F12F1.1; F12F1_1; NITRATE TRANSPORTER 1; NITRATE/CHLORATE TRANSPORTER; NPF6.3; NRT1; NRT1/ PTR family 6.3
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