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Agrisera
NRT2.1/Nitrate transporter 2.1 Polyclonal Antibody
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NRT2.1/Nitrate transporter 2.1 Antibody (AS12-2612) in WB
50 µg of microsomal protein from wild type Arabidopsis thaliana (Col 0) roots at various stages of development (21 and 42 days) and from NRT 2.1 deletion mutant (KO-NRT 2.1) were solubilized with LaemmLi x 2 sample buffer and were separated on 12% SDS-PAGE gel. The proteins were transferred (tank transfer, 100 V, 1 h) to a PVDF membrane (Immobilon-P, 0.45 um, Millipore). Blot was blocked for 2 h with agitat... View More
产品信息
AS12-2612
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NRT2.1 C-terminal sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
NRT2.1 encodes a high-affinity nitrate transporter that is predominantly expressed in roots and localizes mainly to the plasma membrane of root epidermal and cortical cells, positioning it at the soil-facing interface for nitrate acquisition. At the protein level, NRT2.1 is a polytopic membrane transporter (predicted to comprise multiple transmembrane helices typical of secondary transporters) that can exist in membranes as a monomer and as part of higher-molecular-weight complexes, and its efficient accumulation and correct targeting to the plasma membrane strictly depend on association with the partner protein NAR2.1 (also termed AtNRT3.1), consistent with an obligate two-component high-affinity uptake system. Functionally, NRT2.1 is a principal component of the root nitrate high-affinity transport system operating at low external nitrate, thereby supporting nitrogen nutrition under limiting conditions; its activity is subject to post-translational control, including phosphorylation-dependent modulation (e.g., at serine 501) that can inactivate transport without necessarily altering NRT2.1 abundance or its interaction with NAR2.1. Beyond transport, genetic evidence indicates NRT2.1 also contributes to integrating nutritional cues with root system architecture, influencing lateral root initiation in a manner that can be partly uncoupled from net nitrate uptake.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ACH1; AT1G08090; ATNRT2.1; ATNRT2:1; LATERAL ROOT INITIATION 1; LIN1; NITRATE TRANSPORTER 2.1; nitrate transporter 2:1; NRT2; NRT2.1; NRT2:1; NRT2;1AT; T6D22.17; T6D22_17
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