Invitrogen

NSUN4 Polyclonal Antibody

查看其他7个NSUN4抗体

抗体检测数据 (5)

NSUN4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NSUN4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

NSUN4 Antibody (PA5-31695) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NSUN4 (Product # PA5-31695) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

NSUN4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NSUN4 Antibody in Western Blot (WB)

NSUN4 Antibody in Western Blot (WB)

NSUN4 Antibody in Western Blot (WB)

NSUN4 Antibody in Western Blot (WB)

Product Image Gallery

NSUN4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NSUN4 (Product # PA5-31695) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

NSUN4 Antibody in Western Blot (WB) — Western Blot using NSUN4 Polyclonal Antibody (Product # PA5-31695). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. NSUN4 Polyclonal Antibody (Product # PA5-31695) diluted at 1:1,000.

NSUN4 Antibody in Western Blot (WB) — Western Blot using NSUN4 Polyclonal Antibody (Product # PA5-31695). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 Polyclonal Antibody (Product # PA5-31695) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

NSUN4 Antibody in Western Blot (WB) — Western Blot analysis of NSUN4 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NSUN4 Polyclonal Antibody (Product # PA5-31695) at a dilution of 1:500.

NSUN4 Antibody in Western Blot (WB) — Western Blot analysis of NSUN4 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NSUN4 Polyclonal Antibody (Product # PA5-31695) at a dilution of 1:500.

产品信息

PA5-31695

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 226 of Human NSUN4

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2549168

产品详细信息

Recommended positive controls: HeLa, HepG2, HCT116, mouse liver, rat liver.

Predicted reactivity: Human (99%), Mouse (84%), Rat (81%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NSUN4 is a 5-methylcytosine rRNA methytransferase that is involved in mitochondiral ribosome assembly. More specifically NSUN4 is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA. This function is independent of MTREFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). NSUN4 is targeted to LSU by MTREFD2/MTERF4 and is involved in the final step in ribosome biogenesis to ensure that SSU and LSU are assembled.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 5-methylcytosine rRNA methyltransferase NSUN4; 5-methylcytosine tRNA methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; NOL1/NOP2/Sun domain family, member 4; NOP2/Sun domain family, member 4; putative methyltransferase NSUN4; RP4-603I14.2; Sperm head and tail associated protein

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基因别名: 2310010O12Rik; 2810405F18Rik; AI507900; NSUN4; RGD1559652; SHTAP

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UniProt ID: (Human) Q96CB9, (Mouse) Q9CZ57

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Entrez Gene ID: (Human) 387338, (Rat) 298426, (Mouse) 72181

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protein binding methyltransferase activity small ribosomal subunit rRNA binding molecular_function rRNA (adenine-N6,N6-)-dimethyltransferase activity RNA binding protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity rRNA binding methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity RNA methyltransferase

参与通路

rRNA methylation mature ribosome assembly positive regulation of mitochondrial translation biological_process methylation rRNA processing ribosome biogenesis