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Invitrogen
NSUN4 Monoclonal Antibody (4G3)
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图: 1 / 2
NSUN4 Antibody (MA5-42533) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of NSUN4 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with NSUN4 Monoclonal Antibody (Product # MA5-42533) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-42533
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4G3
抗原
Recombinant protein within human NSUN4 aa 1-384.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
2 mg/mL
规格
200 µg
纯化类型
Protein G
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: MCF-7 cell lysates, MCF-7.
Subcellular Location: Mitochondrion.
靶标信息
NSUN4 is a 5-methylcytosine rRNA methytransferase that is involved in mitochondiral ribosome assembly. More specifically NSUN4 is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA. This function is independent of MTREFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). NSUN4 is targeted to LSU by MTREFD2/MTERF4 and is involved in the final step in ribosome biogenesis to ensure that SSU and LSU are assembled.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5-cytosine rRNA methyltransferase NSUN4; 5-cytosine tRNA methyltransferase NSUN4; mRNA cytosine C(5)-methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; RP4-603I14.2
基因别名: NSUN4
Entrez Gene ID: (Human) 387338
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