Invitrogen

NUDCD2 Polyclonal Antibody

查看其他5个NUDCD2抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

NUDCD2 Antibody in Immunocytochemistry (ICC/IF)

NUDCD2 Antibody in Immunocytochemistry (ICC/IF)

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NUDCD2 Antibody (PA5-118128) in ICC/IF

Immunofluorescence staining of NUDCD2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NUDCD2 Polyclonal Antibody (Product # PA5-118128, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

NUDCD2 Antibody in Immunocytochemistry (ICC/IF)

NUDCD2 Antibody in Western Blot (WB)

NUDCD2 Antibody in Immunoprecipitation (IP)

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NUDCD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of NUDCD2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NUDCD2 Polyclonal Antibody (Product # PA5-118128, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

NUDCD2 Antibody in Western Blot (WB) — Western Blot using NUDCD2 Polyclonal Antibody (Product # PA5-118128) at 1:500 dilution. Lane A: K562 Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa.

NUDCD2 Antibody in Immunoprecipitation (IP) — NUDCD2 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 4 µL with NUDCD2 Polyclonal Antibody (Product # PA5-118128) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: NUDCD2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa.

产品信息

PA5-118128

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

免疫沉淀 (IP)

1-5 µL/mg of lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human NUDCD2 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

NUDCD2 may regulate the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: NudC domain-containing protein 2; NudC-like protein 2

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基因别名: NUDCD2; NudCL2

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UniProt ID: (Human) Q8WVJ2

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Entrez Gene ID: (Human) 134492

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protein binding microtubule or microtubule-binding cytoskeletal protein