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Invitrogen
NY-BR-1 Polyclonal Antibody
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NY-BR-1 Antibody (PA5-101251) in ICC/IF
Immunocytochemistry analysis of NY-BR-1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NY-BR-1 polyclonal antibody (Product # PA5-101251) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-101251
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ANKRD30A(Accession Q9BXX3), corresponding to amino acid residues H1102-L1152.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total AN30A.
靶标信息
NY-BR-1 is a novel differentiation marker predominantly expressed in normal breast and breast cancer. It has been shown by Immunohistochemistry (IHC) that NY-BR-1 was present solely in ductal epithelium of normal breast tissue. Invasive carcinoma of the breast and carcinoma in situ were positive for NY-BR-1, whereas most other tumors and normal tissues are negative, with the exceptions of one-third of sweat gland carcinomas and around two percent of prostate carcinoma. NY-BR-1 is reported to express more frequently in estrogen-positive and lymph node-negative primary carcinomas, and more commonly in grade 1 than in grade 2 or grade 3. In a study by Varga et al (2006) forty-nine percent of lymph node metastasis were shown to be positive.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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