Invitrogen

Nanog Polyclonal Antibody, PE

Name: Nanog Polyclonal Antibody, PE
Brand: Invitrogen
SKU: PA5-46891

3 篇参考文献

查看其他35个Nanog抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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Nanog Antibody (PA5-46891) in Flow

Flow cytometry of Nanog in Three-day Endoderm-differentiated BG01V human embryonic stem cells (upper image) or untreated BG01V human embryonic stem cells (lower image). Samples were incubated in Nanog polyclonal antibody (Product # PA5-46891) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.

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Immunogen Image Nanog Polyclonal Antibody, PE

产品信息

PA5-46891

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

10 µL per million cells

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

C. elegans, Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant human Nanog Trp153-Val305

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偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Buffered saline solution with ≤ 0.5 BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2577239

产品详细信息

In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.

靶标信息

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。