Invitrogen

Nanog Polyclonal Antibody

2个已发表图片
4 篇参考文献
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说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持

产品信息

PA5-85110

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:3,000
查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

-
查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent
-

免疫组化（冰冻） (IHC (F))

Assay-dependent
-

免疫细胞化学 (ICC/IF)

1:100-1:1,000
查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1:1,000-1:10,000
-

免疫沉淀 (IP)

1:100-1:500
-
产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2792258

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Chimpanzee (98%).

Positive Control: HeLa nucleus, NIH-3T3, mouse ESC.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48

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基因别名: 2410002E02Rik; Ecat4; ENK; NANOG

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UniProt ID: (Human) Q9H9S0, (Mouse) Q80Z64

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Entrez Gene ID: (Human) 79923, (Mouse) 71950

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