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Agrisera
NdhH/NAD(P)H-quinone oxidoreductase subunit H (chloroplastic) Polyclonal Antibody
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NdhH/NAD(P)H-quinone oxidoreductase subunit H (chloroplastic) Antibody (AS16-4065) in WB
Total proteins of Arabidopsis thaliana leaf were precipitated with 10% TCA, washed with acetone and solubilized in a buffer with 8 M urea, 100 m M Tris-HCL p H 7.5,1 m M EDTA, 2% SDS. 10 µg of TCA precipitates were separated in a Bio-Rad TGX 4-20% precast gels and blotted 1 h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 8% milk in TTBS for 30 min. at room temperature (RT) with agi... View More
产品信息
AS16-4065
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NdhH protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Setaria viridis, Zea mays
靶标信息
NdhH is a plastid-encoded core subunit of the chloroplast NADH dehydrogenase-like (NDH) complex that localizes to the thylakoid membrane, where it forms part of the membrane arm near the plastoquinone (PQ) reduction site. In land-plant chloroplasts, NdhH is typically a ~390 aa, strongly hydrophobic integral membrane protein predicted to contain multiple transmembrane helices and conserved sequence features shared with cyanobacterial NDH-1/respiratory complex I homologs; structural analyses of the plant NDH-PSI supercomplex place NdhH adjacent to other membrane-arm components and contributing, via extramembranous loops, to shaping the PQ-binding pocket region. Functionally, NdhH supports NDH-dependent cyclic electron flow around photosystem I by enabling ferredoxin-dependent electron transfer into the PQ pool coupled to vectorial proton translocation across the thylakoid membrane, thereby strengthening the proton motive force to increase ATP production and helping balance the ATP:NADPH output of photosynthesis and protect the photosynthetic apparatus under fluctuating or stress conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: NADH dehydrogenase subunit 7; NADH-plastoquinone oxidoreductase subunit 7; ndhH
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