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Agrisera
NdhS/NAD(P)H-quinone oxidoreductase subunit S (chloroplastic) Polyclonal Antibody
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NdhS/NAD(P)H-quinone oxidoreductase subunit S (chloroplastic) Antibody (AS16-4066) in WB
Arabidopsis thaliana leaves from wildtype and ndho mutant were frozen in liquid nitrogen. Leaf tissue was homogenized in ice-cold isolation buffer (330 mm Suc, 25 mm HEPES-KOH, p H 7.4,10 mm Mg Cl 2, and 10 mm Na F) and filtered through Miracloth. The filtrate was centrifuged at 6,000 g for 5 min at 4°C. The thylakoid pellet was resuspended in 25 mm HEPES-KOH, p H 7.4,10 mm Mg Cl 2, and 10 mm Na F, centrifu... View More
产品信息
AS16-4066
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NdhS sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Hordeum vulgare
靶标信息
NdhS encodes a small, nucleus-encoded accessory subunit of the chloroplast NADH dehydrogenase-like (NDH) complex that localizes to thylakoid membranes on the stromal side, where it contributes to the hydrophilic, stroma-exposed arm of NDH. Structurally, NdhS belongs to a conserved NdhS protein family found in oxygenic photosynthetic lineages and is thought to act primarily through protein-protein interactions rather than catalysis, helping organize the electron-donor interface of the complex; specifically, biochemical and genetic evidence supports a role for NdhS in forming or stabilizing a ferredoxin interaction site required for high-affinity ferredoxin binding. Functionally, by promoting efficient ferredoxin-dependent reduction of plastoquinone, NdhS supports NDH activity in cyclic electron transport around photosystem I and chlororespiration, processes that augment proton motive force across the thylakoid membrane and thereby help tune ATP production and protect photosystems under fluctuating or high-light conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CRR31; ssl0352
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