Invitrogen

Nectin 2 Polyclonal Antibody

Name: Nectin 2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143985

查看其他32个Nectin 2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (6)

Nectin 2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

Nectin 2 Antibody (PA5-143985) in ICC/IF

Immunocytochemistry analysis of Nectin 2 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Nectin 2 Polyclonal antibody (Product # PA5-143985) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropr... View More

Nectin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nectin 2 Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Nectin 2 Polyclonal Antibody

产品信息

PA5-143985

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Nectin 2/NECTIN2 recombinant protein (Position: Q32-E348).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3075199

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, human HEK293 whole cell. IHC: human mammary cancer tissue, rat testis tissue. ICC/IF: Hela cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD112; Herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; Nectin cell adhesion molecule 2; Nectin-2; poliovirus receptor related 2; poliovirus receptor-like 2; poliovirus receptor-related 2 (herpesvirus entry mediator B); Poliovirus receptor-related protein 2

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基因别名: CD112; HVEB; NECTIN2; PRR2; PVRL2; PVRR2

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UniProt ID: (Human) A8K5L5

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Entrez Gene ID: (Human) 5819, (Rat) 308417

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功能

virus receptor activity receptor binding protein binding coreceptor activity identical protein binding protein homodimerization activity protein heterodimerization activity cell adhesion molecule binding molecular_function

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