Invitrogen

Nephrin Monoclonal Antibody (C8)

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Nephrin Antibody (MA5-41644) in WB

Western blot analysis of Nephrin in Human 293T cell lysate. Samples were incubated with monoclonal antibody (Product # MA5-41644) at a dilution of 1 µg/mL, followed by HRP-Linked Caprine Anti-Mouse IgG using a dilution of 0.2 µg/mL.

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产品信息

MA5-41644

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.01-2 µg/mL

免疫细胞化学 (ICC/IF)

5-20µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

抗原

Recombinant protein Nephrin. The antigen corresponds to amino acid range 27-234 of the target protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2899126

靶标信息

Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.

仅用于科研。不用于诊断过程。未经明确授权不得转售。