Invitrogen

Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488, eBioscience™

Name: Nestin Monoclonal Antibody (10C2), Alexa Fluor\u2122 488, eBioscience\u2122
Brand: Invitrogen
SKU: 53-9843-82

10个已发表图片

13 篇参考文献

查看其他41个Nestin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (10)

Nestin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

Nestin Antibody (53-9843-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized SKNSH cells using 10 µg/mL of Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or 10 µg/mL Anti-Human Nestin Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Nestin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Nestin Antibody in Immunohistochemistry (Frozen), Immunocytochemistry (IHC (F), ICC/IF)

Nestin Antibody in Immunohistochemistry (IHC)

Nestin Antibody in Flow Cytometry (Flow)

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产品信息

53-9843-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

Assay-Dependent

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免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

10C2

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1834346

产品详细信息

Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.

Applications Reported: This 10C2 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining, and flow cytometric analysis.

Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

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