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Invitrogen
Nestin Monoclonal Antibody (10C2), DyLight™ 650
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FIGURE: 1 / 1
Nestin Antibody (MA5-16103) in Flow
Flow cytometry analysis of Nestin in SH-SY5Y cells. Samples were incubated with Nestin monoclonal antibody (Product # MA5-16103) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. An intracellular stain was performed with primary antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
Product Details
MA5-16103
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
10C2
Immunogen
A fragment of the human nestin protein corresponding to residues 1464-1614.
Conjugate
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
1.58 mg/mL
Purification
Protein G
Storage buffer
50mM sodium borate
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody does not cross-react with rodent or rabbit.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; Nestin
Gene Aliases: AA166324; C78523; ESTM46; Nbla00170; NES
UniProt ID: (Human) P48681, (Mouse) Q6P5H2
Entrez Gene ID: (Rabbit) 100008641, (Human) 10763, (Rat) 25491, (Mouse) 18008
G2/M transition of mitotic cell cycle
nervous system development
brain development
response to ionizing radiation
neuron differentiation
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
response to nutrient levels
negative regulation of protein binding
response to drug
negative regulation of catalytic activity
negative regulation of neuron apoptotic process
cell projection morphogenesis
stem cell proliferation
positive regulation of neural precursor cell proliferation
multicellular organism development
central nervous system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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