Invitrogen

Neuroligin 1 Monoclonal Antibody (N97A/31), PerCP

查看其他30个Neuroligin 1抗体

问题与答案

问题与答案

抗体检测数据 (3)

Neuroligin 1 Antibody in Immunocytochemistry (ICC/IF)

Neuroligin 1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

Neuroligin 1 Antibody (MA5-45687) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Neuroligin 1 monoclonal antibody (Product # MA5-45687) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000... View More

Neuroligin 1 Antibody in Immunocytochemistry (ICC/IF)

Neuroligin 1 Antibody in Immunocytochemistry (ICC/IF)

Neuroligin 1 Antibody in Western Blot (WB)

Product Image Gallery

Neuroligin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Neuroligin 1 monoclonal antibody (Product # MA5-45687) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 1 antibody. (D) Composite.

Neuroligin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Neuroligin 1 monoclonal antibody (Product # MA5-45687) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1 antibody (D) Composite.

Neuroligin 1 Antibody in Western Blot (WB) — Western Blot analysis of Mouse Brain Membrane showing detection of ~120 kDa Neuroligin 1 protein. Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Blocking: 2% BSA and 2% Skim Milk in 1X TBST. Samples were incubated with Neuroligin 1 monoclonal antibody (Product # MA5-45687) at 1:200 for 16 hours at 4°C, followed by Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~120 kDa.

Immunogen image

Neuroligin 1 Monoclonal Antibody (N97A/31), PerCP

Immunogen Image Neuroligin 1 Monoclonal Antibody (N97A/31), PerCP

产品信息

MA5-45687

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化 (IHC)

Assay-dependent

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

N97A/31

抗原

Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1.

查看免疫原

偶联物

PerCP PerCP PerCP

激发/发射光谱

482/675 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3.

2 µg/mL of MA5-45687 was sufficient for detection of Neuroligin-1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 120kDa. Does not cross-react with other Neuroligins.

This antibody was formerly sold as clone S97A-31.

靶标信息

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: MGC45115; Neuroligin I; Neuroligin-1; NLG 1

查看更多收起

基因别名: 6330415N05Rik; BB179718; KIAA1070; mKIAA1070; NL1; Nlg1; NLGN1

查看更多收起

UniProt ID: (Human) Q8N2Q7, (Mouse) Q99K10, (Rat) Q62765

查看更多收起

Entrez Gene ID: (Human) 22871, (Mouse) 192167, (Rat) 116647

查看更多收起

receptor activity PDZ domain binding neurexin family protein binding protein dimerization activity cell adhesion molecule binding carboxylic ester hydrolase activity scaffold protein binding protein binding cell adhesion molecule

regulation of respiratory gaseous exchange by neurological system process protein targeting heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules neuron cell-cell adhesion nervous system development synapse assembly positive regulation of circadian sleep/wake cycle, wakefulness synaptic vesicle targeting calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules neuronal signal transduction neuron projection development positive regulation of synaptic transmission, GABAergic protein localization to synapse establishment of protein localization regulation of neuron differentiation rhythmic process cytoskeletal matrix organization at active zone protein homooligomerization protein heterotetramerization positive regulation of filopodium assembly positive regulation of synapse assembly positive regulation of synaptic transmission, glutamatergic long-term synaptic potentiation positive regulation of dendritic spine development negative regulation of dendritic spine morphogenesis terminal button organization synaptic vesicle clustering postsynaptic membrane assembly presynaptic membrane assembly AMPA glutamate receptor clustering NMDA glutamate receptor clustering neurexin clustering involved in presynaptic membrane assembly postsynaptic density protein 95 clustering receptor localization to synapse positive regulation of ruffle assembly positive regulation of synaptic vesicle endocytosis positive regulation of protein localization to synapse positive regulation of intracellular signal transduction positive regulation of synaptic vesicle exocytosis regulation of N-methyl-D-aspartate selective glutamate receptor activity regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity positive regulation of excitatory postsynaptic potential positive regulation of synaptic vesicle clustering cell adhesion synaptic vesicle transport modulation of synaptic transmission synapse organization inhibitory postsynaptic potential metabolic process neuronal ion channel clustering