Search
Invitrogen
Nhe-1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 5
Nhe-1 Antibody (PA5-115917) in ICC/IF
Immunocytochemistry analysis of Nhe-1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115917) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-115917
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SLC9A1(Accession P19634), corresponding to amino acid residues R516-E566.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total SLC9A1.
靶标信息
The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Na(+)/H(+) exchanger 1; Na+/H+ antiporter; Na+/H+, amiloride sensitive; Na-Li countertransporter; NHE-1; plasma membrane antiporter; hemopoietic NHE1; APNH; protein phosphatase 1, regulatory subunit 143; slow-wave epilepsy; Solute carrier family 9 (sodium/hydrogen exchanger 1), antiporter, Na+/H+, (amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 member 1; solute carrier family 9, member 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1; unnamed protein product
基因别名: APNH; LIKNS; mir-5122; Mir5122; NHE-1; NHE1; PPP1R143; swe
UniProt ID: (Human) P19634, (Mouse) Q61165, (Rat) P26431
Entrez Gene ID: (Human) 6548, (Mouse) 20544, (Rat) 24782
calcium ion binding
protein binding
phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
antiporter activity
sodium:proton antiporter activity
potassium:proton antiporter activity
protein phosphatase 2B binding
protein binding, bridging
identical protein binding
calcium-dependent protein binding
binding, bridging
regulation of the force of heart contraction
cation transport
sodium ion transport
cellular sodium ion homeostasis
regulation of pH
response to acidic pH
positive regulation of cardiac muscle hypertrophy
regulation of cardiac muscle contraction by calcium ion signaling
cell differentiation
hyaluronan catabolic process
positive regulation of cell growth
cellular response to insulin stimulus
positive regulation of mitochondrial membrane permeability
response to muscle stretch
sodium ion export from cell
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of action potential
positive regulation of transcription from RNA polymerase II promoter
protein oligomerization
regulation of intracellular pH
cardiac muscle cell differentiation
transmembrane transport
cellular response to cold
positive regulation of calcineurin-NFAT signaling cascade
cellular response to antibiotic
cellular response to electrical stimulus
cellular response to hypoxia
cellular response to acidic pH
potassium ion transmembrane transport
cellular response to epinephrine stimulus
cardiac muscle cell contraction
regulation of the force of heart contraction by cardiac conduction
sodium ion import across plasma membrane
positive regulation of the force of heart contraction
positive regulation of intracellular signal transduction
hydrogen ion transmembrane transport
ion transport
cell migration
maintenance of cell polarity
sodium ion transmembrane transport
regulation of stress fiber assembly
regulation of focal adhesion assembly
cellular response to mechanical stimulus
regulation of cardiac muscle cell membrane potential
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
