Invitrogen

Nodal Polyclonal Antibody

查看其他24个Nodal抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (5)

Nodal Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nodal Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Nodal Antibody (PA5-28486) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Nodal (Product # PA5-28486) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Nodal Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nodal Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nodal Antibody in Western Blot (WB)

Nodal Antibody in Western Blot (WB)

Nodal Antibody in ELISA (ELISA)

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Nodal Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human breast cancer, using Nodal (Product # PA5-28486) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Nodal Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of Nodal was performed in paraffin-embedded rat brain tissue using Nodal Polyclonal Antibody (Product # PA5-28486) at a dilution of 1:500.

Nodal Antibody in Western Blot (WB) — Western Blot using Nodal Polyclonal Antibody (Product # PA5-28486). Sample (20 µg of whole cell lysate). Lane A: Human ESC. 10% SDS PAGE. Nodal Polyclonal Antibody (Product # PA5-28486) diluted at 1:1,000.

Nodal Antibody in Western Blot (WB) — Western Blot using Nodal Polyclonal Antibody (Product # PA5-28486). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Nodal Polyclonal Antibody (Product # PA5-28486) diluted at 1:500.

Nodal Antibody in ELISA (ELISA) — ELISA detection of NODAL using Nodal Polyclonal Antibody (Product # PA5-28486) for capture at a concentration of 5 µg/mL and a NODAL Polyclonal Antibody for detection at a concentration of 1.5 µg/mL.

产品信息

PA5-28486

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

酶联免疫吸附实验 (ELISA)

1:1,000-1:10,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Recommended positive controls: Human ESC.

Predicted reactivity: Mouse (91%), Rat (92%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NODAL is an important TGF-beta family protein in cellular differentiation. While constitutively expressed in many developing tissues, NODAL is problematically associated with the differentiation and development of many cancers. The expression of NODAL is regulated by a Notch signaling pathway, which when disrupted contributes to the aggressive behavior of metastatic cancer cells, particularly in melanoma. The abundance of NODAL expression in melanoma can help determine the stage of cancer and metastatic type/progression when tested in IHC. NODAL interactions with Cripto also predispose toward tumor progression. Overexpression of NODAL is also associated with preeclamptic placenta.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: early embryo mesoderm formation; MGC138230; Nodal; Nodal homolog; transgenic lethal 413.d

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基因别名: HTX5; NODAL; Tg.413d

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UniProt ID: (Human) Q96S42, (Mouse) P43021

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Entrez Gene ID: (Human) 4838, (Rat) 294503, (Mouse) 18119

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cytokine activity transforming growth factor beta receptor binding growth factor activity morphogen activity receptor agonist activity type I activin receptor binding protein binding growth factor

negative regulation of transcription from RNA polymerase II promoter trophectodermal cellular morphogenesis neural fold formation liver development placenta development embryonic placenta development maternal placenta development vasculature development heart looping inhibition of neuroepithelial cell differentiation brain development embryonic pattern specification polarity specification of proximal/distal axis regulation of gastrulation positive regulation of vascular endothelial growth factor production negative regulation of cell development positive regulation of pathway-restricted SMAD protein phosphorylation stem cell population maintenance positive regulation of cell-cell adhesion BMP signaling pathway positive regulation of activin receptor signaling pathway floor plate morphogenesis endodermal cell differentiation cell migration involved in gastrulation positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter axial mesodermal cell fate specification mesendoderm development digestive tract morphogenesis embryonic cranial skeleton morphogenesis formation of anatomical boundary positive regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity embryonic process involved in female pregnancy maternal process involved in parturition positive regulation of SMAD protein import into nucleus SMAD protein signal transduction left lung morphogenesis negative regulation of androgen receptor signaling pathway epiblast cell-extraembryonic ectoderm cell signaling involved in anterior/posterior axis specification positive regulation of ERK1 and ERK2 cascade transforming growth factor beta receptor signaling pathway involved in primitive streak formation nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry negative regulation of trophoblast cell migration negative regulation of chorionic trophoblast cell proliferation regulation of stem cell population maintenance in utero embryonic development gastrulation with mouth forming second mesoderm formation trophectodermal cell differentiation determination of left/right symmetry gastrulation nervous system development endoderm development heart development positive regulation of cell proliferation anterior/posterior axis specification anterior/posterior pattern specification regulation of signal transduction cell migration lung development embryonic heart tube development nodal signaling pathway growth regulation of apoptotic process regulation of MAPK cascade cell fate commitment negative regulation of cell differentiation cell development anatomical structure formation involved in morphogenesis tissue morphogenesis digestive system development primitive streak formation positive regulation of receptor activity transforming growth factor beta receptor signaling pathway multicellular organism development