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Agrisera
NpHR/Halorhodopsin Polyclonal Antibody
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NpHR/Halorhodopsin Antibody (AS12-1851) in WB
Following SDS-PAGE (12% Separation Gel) the Blot (PVDF) was stained with Ponceau S (A, lowest panel), divided in two halves, scanned and blocked with 5% not-fat milk powder (Marvel) o. n. at 4 °C. The halves were then incubated separately at RT for 1 h with anti-Np HR or pre-immune serum at the indicated dilutions, rinsed briefly twice with TBS-T (p H 7.4), then washed three times for 15 min, incubated for 1 h at RT with goat anti-rabbit-HRP conjugated secondary antibody (Agrisera, # AS09 602) in a dilution of 1:100,000 and washed as descr... View More
产品信息
AS12-1851
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Natronomonas pharaonis halorhodopsin sequences.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Natronomonas pharaonis
靶标信息
NpHR encodes a microbial rhodopsin from the haloarchaeon Natronomonas pharaonis that is embedded in the cell membrane as a retinylidene protein with seven transmembrane helices (A-G) and an N-terminal amphipathic helix (A') that, together with an extended extracellular loop between helices B and C, forms a cap over the extracellular face of the protein. The chromophore retinal is covalently linked to a conserved lysine in helix G via a protonated Schiff base positioned near a primary chloride-binding site, where the anion is stabilized by polar residues (including Thr126 and Ser130) adjacent to the Schiff base. Structurally, NpHR assembles as a trimer, and light absorption drives retinal isomerization and a photocycle that results in active transport of a halide ion (preferentially chloride) from the extracellular side to the cytoplasmic side. Functionally, this inward light-driven chloride pumping alters the transmembrane electrochemical gradient (hyperpolarizing when expressed in heterologous membranes), and targeted mutagenesis and electrophysiology support a key role for residues near the Schiff base region (e.g., Ser81 influencing Thr126-mediated anion coordination) in enabling efficient chloride migration through the protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eNpHR; eNpHR3.0; halorhodopsin; HR; Natronomonas pharaonis halorhodopsin; pharaonis halorhodopsin; phR
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