Search
Agrisera
NtcA/Global nitrogen regulator Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 1
NtcA/Global nitrogen regulator Antibody (AS12-1873) in WB
5 µg of total protein from Synechocystis sp. PCC 6,803 extracted with PEB were separated on 4-12% SDS-PAGE and blotted 1 h to PVDF. Blots were blocked for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 time... View More
产品信息
AS12-1873
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences.
偶联物
形式
Lyophilized
规格
200 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Synechocystis sp. PCC6803
靶标信息
NtcA is a cytosolic, sequence-specific DNA-binding transcription factor found in cyanobacteria, where it functions as a global regulator coordinating the cellular response to nitrogen availability. It is a CRP-family regulator that forms homodimers; each subunit contains an N-terminal effector-binding domain and a C-terminal helix-turn-helix DNA-binding domain connected by a long alpha-helical linker that helps position the DNA-recognition helices appropriately for promoter binding. NtcA directly controls expression of numerous genes involved in nitrogen uptake and assimilation (including activation of key assimilation functions and autoregulation), and its activity is tuned by the nitrogen starvation signal 2-oxoglutarate, which acts as an allosteric effector that enhances NtcA-DNA binding and promotes transcriptional activation of NtcA-dependent promoters under nitrogen limitation, thereby coupling carbon/nitrogen balance to transcriptional output.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: bifA; VF1
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
