NeoBiotechnologies

Nucleophosmin (Acute Myeloid Leukemia Marker) Monoclonal Antibody (NPM1/3287)

Name: Nucleophosmin (Acute Myeloid Leukemia Marker) Monoclonal Antibody (NPM1\/3287)
Brand: NeoBiotechnologies
SKU: 4869-MSM7-P1ABX

查看其他56个NPM1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (6)

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody (4869-MSM7-P1ABX) in ICC/IF

Immunofluorescence staining of HeLa cells using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/3287) followed by GAM IgG-CF488 (green). Membrane stained with phalloidin (red).

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody in Western Blot (WB)

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody in Flow Cytometry (Flow)

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody in Peptide array (ARRAY)

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody in SDS-PAGE (SDS-PAGE)

Product Image Gallery

Immunogen image

Immunogen Image Nucleophosmin (Acute Myeloid Leukemia Marker) Monoclonal Antibody (NPM1/3287)

产品信息

4869-MSM7-P1ABX

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1-2 µg/mL

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

1-2 µg/10^6 cells

酶联免疫吸附实验 (ELISA)

2-4 µg/mL

肽阵列 (Array)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

NPM1/3287

抗原

Recombinant fragment (around aa185-287) of human NPM1 protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: B23 antibody; MGC104254; mNPM antibody; mNPM1 antibody; NO38 antibody; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128

查看更多收起

基因别名: B23; NPM; NPM1

查看更多收起

UniProt ID: (Human) P06748

查看更多收起

Entrez Gene ID: (Human) 4869

查看更多收起

功能