Invitrogen

Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

Name: Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)
Brand: Invitrogen
SKU: OMA1-03132

高级验证

4个已发表图片

11 篇参考文献

查看其他41个beta Amyloid抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)

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Amyloid Precursor Protein Antibody (OMA1-03132) in ICC/IF

Immunofluorescence analysis of Amyloid Precursor Protein was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1) (Product # OMA1-03132) at 1:200 dilut... View More

Amyloid Precursor Protein Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

产品信息

OMA1-03132

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

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免疫细胞化学 (ICC/IF)

1:200

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酶联免疫吸附实验 (ELISA)

10 µg/mL

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免疫沉淀 (IP)

25 µg/mL*

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产品规格

种属反应

Human, Non-human primate

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

mAbP2-1

抗原

Native, secreted form of APP

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-80°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325526

产品详细信息

OMA1-03132 detects amyloid precursor protein (APP) from human and monkey tissues. This antibody is specific for native, non-denatured protein, and does not cross-react with mouse, rat APP or other APP homologs.

OMA1-03132 has been successfully used in Western blot (non-reducing conditions), immunocytochemistry, immunoprecipitation and ELISA procedures. This antibody does not work in in IHC on paraffin embedded tissues nor does it recognize APP if the samples are boiled in Laemmli (SDS) sample buffer containing a reducing agent such as DTT or β-ME. Can be used in immunoprecipitation utilizing 25 µg/mL of OMA1-03132 linked sepharose.

OMA1-03132 antigen is the native, secreted form of human APP. The epitope recognized by OMA1-03132 maps to residues 104-118 of APP

靶标信息

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer's disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down's syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ABPP; Alzheimer disease; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; Amyloid precursor protein; Amyloid β; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; APP; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTF gamma; CTF-alpha; CVAP; OTTHUMP00000096096; Pan-Abeta; peptidase nexin-II; PN-II; PreA4; PreA4 751; Protease nexin-II; testicular tissue protein Li 2

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基因别名: A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2

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UniProt ID: (Human) P05067

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Entrez Gene ID: (Human) 351

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