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Bethyl Laboratories
OTUB1 Polyclonal Antibody
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FIGURE: 1 / 1
OTUB1 Antibody (A302-918A) in WB
Detection of human and mouse OTUB1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody (Product # A302-918A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody A302-917A, which recognizes an upstream epitope. Detection: Chemiluminescence with e... View More
Product Details
A302-918A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 40 and 80 of human OTU Domain, Ubiquitin Aldehyde Binding 1.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
The product of this gene is a member of the OTU superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme OTUB1; FLJ20113; FLJ40710; hOTU1; MGC111158; MGC4584; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU-domain Ubal-binding 1; otubain 1; Otubain-1; Ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; Ubiquitin-specific-processing protease OTUB1; unnamed protein product
Gene Aliases: AI850305; HSPC263; OTB1; OTU1; OTUB1
UniProt ID: (Human) Q96FW1, (Mouse) Q7TQI3
Entrez Gene ID: (Human) 55611, (Mouse) 107260
adaptive immune response
immune system process
DNA repair
proteolysis
cellular response to DNA damage stimulus
protein deubiquitination
cellular response to nutrient levels
TORC1 signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
protein K48-linked deubiquitination
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
negative regulation of double-strand break repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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