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Invitrogen
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), PE, eBioscience™
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-peptide-bound-to-H-2Kb-Flow-20170216130641.jpg?time=20220826 "OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody in Flow Cytometry (Flow)")
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OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody (12-5743-82) in Flow
Staining of BALB/c (left) or C57BL/6 splenocytes (right) either unpulsed (open histogram) or pulsed with OVA257-264 (SIINFEKL) peptide (filled histogram) with 0.25 µg of Anti-Mouse OVA257-264 (SIINFEKL) peptide bound to H-2Kb PE. Total viable cells were used for analysis.
-peptide-bound-to-H-2Kb-Flow-20170216130641.jpg?time=20220826)
-peptide-bound-to-H-2Kb-Flow-20170216130641.jpg?time=20220826?time=20220826 "OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody (12-5743-82) in Flow")
产品信息
12-5743-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Human,
Mouse,
Yeast
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBio25-D1.16 (25-D1.16)
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.
Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Cells can be pulsed with the SIINFEKL peptide according to the following protocol:
1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM.
2. Incubate cells at 37°C for 2 hours.
3. Wash cells with flow staining buffer.
4. Proceed with cell surface staining as normal.
For additional information see the references listed below.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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