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Invitrogen
Occludin Polyclonal Antibody
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FIGURE: 1 / 62
Occludin Antibody (40-4700) in ICC/IF
Immunofluorescent analysis of occludin Antibody was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Occludin Antibody (Product # 40-4700) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room... View More
Product Details
40-4700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
C. elegans,
Chicken,
Dog,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the C-terminal region of the human occludin protein
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the human occludin protein. On western blots, it identifies the target band at ~65 kDa.
Reactivity has been confirmed with human Caco-2 and HT29, dog MDCK, mouse TCMK, and rat KNRK cell lysates, and mouse kidney, liver, and rat liver homogenates by western blotting, Caco-2 cells by immunofluorescence, and paraffin-embedded human small intestine tissue by immunohistochemistry.
For immunofluorescence using Caco-2 cells, fixation with cold ethanol is recommended. For immunohistochemistry in paraffin-embedded tissues, enzyme digestion with pepsin is required prior to staining.
Target Information
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; tight junction structural protein
Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115
UniProt ID: (Dog) Q28269, (Human) Q16625, (Mouse) Q61146, (Rat) Q6P6T5
Entrez Gene ID: (Dog) 403844, (Human) 100506658, (Mouse) 18260, (Rat) 83497
positive regulation of lamellipodium assembly
positive regulation of microtubule polymerization
bicellular tight junction assembly
positive regulation of wound healing
protein localization to cell leading edge
protein complex assembly
methylation
cell-cell junction organization
S-adenosylhomocysteine metabolic process
S-adenosylmethionine metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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