Search
Invitrogen
Osteopontin Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 1
Osteopontin Antibody (PA5-94926) in WB
Western blot analysis of Osteopontin in, Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SHG-44 whole cell lysates, . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-94926
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin (281-314aa HEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue, human SHG-44 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 44 kDa bone phosphoprotein; bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); calcium oxalate crystal growth inhibitor protein; CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; early T-lymphocyte activation 1 protein; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; lnc-PKD2-2-3; MGC110940; minopontin; nephropontin; OSN-C; OSN-D; osteopontin precursor; osteopontin-like; osteopontin-like protein; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; putative OPN isoform 6; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); Sialoprotein (osteopontin); SPP-1; Spp1; SPP1/CALPHA1 fusion; unnamed protein product; urinar; urinary stone protein; uropontin
基因别名: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; OPN; Opnl; OSP; Ric; Spp-1
UniProt ID: (Human) P10451, (Mouse) P10923, (Rat) P08721
Entrez Gene ID: (Human) 6696, (Mouse) 20750, (Rat) 25353
ossification
osteoblast differentiation
androgen catabolic process
cell adhesion
embryo implantation
cell differentiation
bone mineralization
response to vitamin D
response to macrophage colony-stimulating factor
positive regulation of bone resorption
positive regulation of transcription, DNA-templated
decidualization
response to steroid hormone
negative regulation of collateral sprouting of intact axon in response to injury
calcium ion homeostasis
cellular response to testosterone stimulus
response to 2,3,7,8-tetrachlorodibenzodioxine
positive regulation of estradiol secretion
cellular calcium ion homeostasis
cellular sodium ion homeostasis
signal transduction
positive regulation of cell-substrate adhesion
neutrophil chemotaxis
cellular phosphate ion homeostasis
cellular chloride ion homeostasis
urate biosynthetic process
negative regulation of apoptotic process
cellular response to fluid shear stress
collecting duct development
cellular response to leukemia inhibitory factor
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
