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Invitrogen
OxPhos Human WB Antibody Cocktail
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OxPhos Human WB Antibody (45-8199) in WB
Western blot analysis of OxPhos in mitochondria isolated from MRC5 using an OxPhos Monoclonal antibody cocktail (Product # 45-8199). Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts. In Lanes 1-5 the individual antibodies that make up the cocktail were used to label these samples. In Lane 6 the OxPhos Monoclonal antibody cocktail (Product # 45-8199) was used to label the sample.
产品信息
45-8199
产品规格
种属反应
Human
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Mouse
/ Ig
分类
Cocktail
类型
Antibody
克隆号
Cocktail
偶联物
形式
Liquid
浓度
1.5 mg/mL
纯化类型
IgG fraction
保存液
HEPES buffered saline
内含物
0.02% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This product is a cocktail of multiple OxPhos Mouse anti Human antibodies.
C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I
This is an optimized premixed cocktail suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from human sources. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used.
C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II
C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III
C-IV-II (clone) 12C4F12 -Human Complex IV, subunit II
C-V-alpha (clone) 15H4C4 -Bovine Complex V
靶标信息
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
