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Invitrogen
OxPhos Human WB Antibody Cocktail
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OxPhos Human WB Antibody (45-8199) in WB
Western blot analysis of OxPhos in mitochondria isolated from MRC5 using an OxPhos Monoclonal antibody cocktail (Product # 45-8199). Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts. In Lanes 1-5 the individual antibodies that make up the cocktail were used to label these samples. In Lane 6 the OxPhos Monoclonal antibody cocktail (Product # 45-8199) was used to label the sample.
产品信息
45-8199
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ Ig
分类
Cocktail
类型
Antibody
克隆号
Cocktail
偶联物
形式
Liquid
浓度
1.5 mg/mL
纯化类型
IgG fraction
保存液
HEPES buffered saline
内含物
0.02% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Contains 5 monoclonal antibodies, one each against Complex I subunit [NDUFB8 (20E9DH10C12)], Complex II subunit 30kDa [SDHB (21A11AE7)], Complex III subunit Core 2 [UQCRC2 (13G12AF12BB11)], Complex IV subunit II [MTCO2 (12C4F12)], and ATP synthase subunit alpha [ATP5A (15H4C4)] as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.
靶标信息
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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