Invitrogen

P-Selectin Monoclonal Antibody (HI62P)

Name: P-Selectin Monoclonal Antibody (HI62P)
Brand: Invitrogen
SKU: MA5-44008

查看其他45个P-Selectin (CD62P)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

P-Selectin Antibody in Western Blot (WB)

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P-Selectin Antibody (MA5-44008) in WB

Western blotting analysis of P-Selectin in RIPA buffer extracts of thrombocytes, leukocytes, and Jurkat cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel. Nitrocellulose membrane blot was probed with P-Selectin monoclonal antibody (Product # MA5-44008) using a dilution of 2 µg/mL followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/mL) was used as the loading control (red). Multiplex fluorescent Western blot detection... View More

P-Selectin Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image P-Selectin Monoclonal Antibody (HI62P)

产品信息

MA5-44008

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

流式细胞分析 (Flow)

1-5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

HI62P

抗原

Human platelets

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2912940

产品详细信息

This antibody recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

靶标信息

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)

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基因别名: CD62; CD62P; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP

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UniProt ID: (Human) P16109

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Entrez Gene ID: (Human) 6403

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功能

lipopolysaccharide binding glycoprotein binding protein binding heparin binding sialic acid binding fucose binding glycosphingolipid binding calcium-dependent protein binding oligosaccharide binding

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