Invitrogen

P-cadherin Monoclonal Antibody (PCD-1)

Name: P-cadherin Monoclonal Antibody (PCD-1)
Brand: Invitrogen
SKU: 13-2000Z

11个已发表图片

34 篇参考文献

查看其他24个P-cadherin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

已发表图片 (11)

P-cadherin Antibody in Immunohistochemistry (IHC)

图: 1 / 11

P-cadherin Antibody (13-2000Z) in IHC

Current issues in molecular biology 2023 - Representative microphotographs of immunohistochemical staining using antibodies against E-cadherin (E-cad) (DAKO, M3612, NCH-38) ( A ), E-cadherin (ProteinTech, 20874-1-AP) ( B ), N-cadherin (N-cad) ( C ), P-cadherin (P-cad) (Santa Cruz, sc-74545) ( D ), beta-catenin ( E ), and vimentin ( F ). Insets of ( A - F ) show enlarged images of microphotographs. Images from cases 1 ( F ), 3 ( C ), 5 ( E ), 6 ( A )... View More

P-cadherin Antibody in Immunocytochemistry (ICC/IF)

P-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

P-cadherin Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

P-cadherin Antibody in Immunohistochemistry (Frozen) (IHC (F))

P-cadherin Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image P-cadherin Monoclonal Antibody (PCD-1)

产品信息

13-2000Z

应用

建议稀释比

已发表文章

免疫印迹 (WB)

10 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看10篇已发表文章 10篇已发表文章

免疫组化（石蜡） (IHC (P))

查看4篇已发表文章 4篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

抑制试剂 (IA)

60 µg/mL

其他PubMed (Misc)

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Bovine, Fish, Hamster, Human, Mouse, Rat, Sheep, Tag

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

PCD-1

抗原

Mouse endoderm cell line PSA5-E.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

2.0 mg/mL

纯化类型

Ion-exchange chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533006

产品详细信息

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell-line with spleen cells of Donryu rat after immunization with mouse endoderm cell line PSA5-E.

Specificity: This antibody specifically reacts with mouse P-cadherin. This antibody inhibits P-cadherin-dependent cell-cell contact.

Cross reactivity: This antibody does not react with human or chicken P-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µl or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Ca2+ should be contained in the buffers to stabilize cadherin antigen.

靶标信息

P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.

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