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Invitrogen
Tyrosine Hydroxylase Polyclonal Antibody
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FIGURE: 1 / 17
Tyrosine Hydroxylase Antibody (PA1-4679) in ICC/IF
Immunofluorescence analysis of Anti-Tyrosine Hydroxylase Polyclonal Antibody (Product # PA1-4679) was performed using 70% confluent log phase PC-12 cells treated with 5 µg/mL dexamethasone for 6 days. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Anti-... View More
Product Details
PA1-4679
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Many,
Mouse,
Rat
Published species
C. elegans,
Dog,
Human,
Mouse,
Rat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma
Conjugate
Form
Liquid
Concentration
0.15 mg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
This antibody is specific for the ~60 kDa Tyrosine Hydroxylase in Western blots of rat brain extracts.
Target Information
Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase
Gene Aliases: DYT14; DYT5b; TH; The; TYH
UniProt ID: (Human) P07101, (Mouse) P24529, (Rat) P04177
Entrez Gene ID: (Human) 7054, (Mouse) 21823, (Rat) 25085
tyrosine 3-monooxygenase activity
protein binding
ferrous iron binding
ferric iron binding
amino acid binding
oxygen binding
enzyme binding
protein domain specific binding
tetrahydrobiopterin binding
dopamine binding
monooxygenase activity
iron ion binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
metal ion binding
response to hypoxia
synaptic transmission, dopaminergic
dopamine biosynthetic process from tyrosine
heart development
visual perception
learning
memory
mating behavior
locomotory behavior
regulation of heart contraction
aromatic amino acid family metabolic process
organ morphogenesis
aminergic neurotransmitter loading into synaptic vesicle
neurotransmitter biosynthetic process
dopamine biosynthetic process
epinephrine biosynthetic process
norepinephrine biosynthetic process
catecholamine biosynthetic process
eye photoreceptor cell development
eating behavior
response to ethanol
embryonic camera-type eye morphogenesis
oxidation-reduction process
response to amphetamine
heart morphogenesis
fatty acid metabolic process
sphingolipid metabolic process
sensory perception of sound
response to water deprivation
response to light stimulus
response to herbicide
response to salt stress
anatomical structure morphogenesis
response to zinc ion
multicellular organism aging
response to activity
glycoside metabolic process
phthalate metabolic process
cerebral cortex development
response to nutrient levels
response to estradiol
response to lipopolysaccharide
isoquinoline alkaloid metabolic process
social behavior
cellular response to drug
response to isolation stress
response to immobilization stress
terpene metabolic process
circadian sleep/wake cycle
response to peptide hormone
pigmentation
response to ether
response to pyrethroid
response to corticosterone
response to electrical stimulus
phytoalexin metabolic process
cellular response to manganese ion
cellular response to nicotine
cellular response to glucose stimulus
cellular response to growth factor stimulus
response to metal ion
response to organic cyclic compound
response to insecticide
response to nicotine
response to drug
response to steroid hormone
cognition
protein homotetramerization
response to growth factor
cellular response to alkaloid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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