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Invitrogen
PAG1 Polyclonal Antibody
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图: 1 / 4
PAG1 Antibody (PA5-115714) in ICC/IF
Immunocytochemistry analysis of PAG1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115714). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-115714
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PAG1(Accession Q9NWQ8), corresponding to amino acid residues V108-G158.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total PAG.
靶标信息
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Csk binding protein; Csk-binding protein; phosphoprotein associated with glycosphingolipid microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; phosphoprotein transmembrane adaptor 1; phosphoprotein-associated with GEMs; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp
基因别名: CBP; F730007C19Rik; PAG; PAG1
UniProt ID: (Rat) Q9JM80, (Mouse) Q3U1F9
Entrez Gene ID: (Rat) 64019, (Mouse) 94212
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