Invitrogen

PAQR3 Polyclonal Antibody

Name: PAQR3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-24654

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2 篇参考文献

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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PAQR3 Antibody (PA5-24654) in WB

Western blot analysis of PAQR3 in WiDr cell line lysates. Samples were incubated with PAQR3 polyclonal antibody (Product # PA5-24654). Lysates: 35 µg/lane. PAQR3 (arrow).

Product Image Gallery

PAQR3 Antibody — Figure 2 PAQR3 modulates ATG14L-linked VPS34 lipid kinase activity A-C HeLa cells stably expressing GFP-DFCP1 were transfected with or without PAQR3-specific shRNA. GFP signals were observed in these cells before or after glucose starvation (GS, shown in A). The knockdown efficiency of PAQR3 is shown in (B), and the statistics of GFP-positive puncta per cell is illustrated in (C). Scale bar: 10 µm. D GFP-WIPI1 was transfected into WT or PAQR3-deficient MEFs. Twenty-four hours later, the cells were incubated under GS for 4 h, followed by fluorescence observation. Scale bar: 10 µm. E Different VPS34 complexes of WT or PAQR3 knockout HeLa cells were immunoprecipitated by ATG14L and UVRAG antibodies, respectively, in normal medium (NM) or GS. Then, PI(3)P level was determined by a quantitative ELISA. The PI(3)P level was normalized to the amount of ATG14L or UVRAG used in the assay. F HeLa cells infected with control or PAQR3-overexpressed lentivirus were treated with GS for 4 h. PI(3)P levels were determined as in Fig 2E.G, H PAQR3 knockout HeLa cells were transfected with plasmids of multiple PAQR3 deletion mutants. At 24 h after transfection, the cell lysates were used to immunoprecipitate VPS34 complex by ATG14L antibody, followed by immunoblotting. The activity of ATG14L-linked VPS34 activity was detected by a quantitative ELISA. The PI(3)P level was normalized to the amount of ATG14L. Data information: Values are presented as mean ± SD (n = 5; **P < 0.01; ***P < 0.001; ns, not significant). {KD}

Immunogen image

Immunogen Image PAQR3 Polyclonal Antibody

产品信息

PA5-24654

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫沉淀 (IP)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 242-270 amino acids from the C-terminal region of human PAQR3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2542154

产品详细信息

This antibody is predicted to react with mouse based on sequence homology.

靶标信息

PAQR3 is function as a spatial regulator of RAF1 kinase by sequestrating it to the Golgi.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Progestin and adipoQ receptor family member 3; Progestin and adipoQ receptor family member III; Raf kinase trapping to Golgi; RKTG

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基因别名: PAQR3; RKTG

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UniProt ID: (Human) Q6TCH7

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Entrez Gene ID: (Human) 152559

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功能

receptor activity G-protein coupled receptor

参与通路

MAPK cascade negative regulation of protein phosphorylation negative regulation of neuron projection development negative regulation of peptidyl-serine phosphorylation protein localization to Golgi apparatus negative regulation of MAP kinase activity

PAQR3 Polyclonal Antibody

PAQR3 Antibody (PA5-24654) in WB

产品信息

PA5-24654

免疫印迹 (WB)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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